Thermo Scientific™ Pierce Protein Concentrators are disposable centrifugal devices for high-performance concentration, diafiltration or buffer-exchange of biological samples.
Pierce Protein Concentrators effectively combine simplicity, speed, capacity and recovery to yield enhanced concentration, purification and separation of proteins, even with dilute protein samples. The high-performance regenerated cellulose membranes in these 150K molecular-weight cutoff (MWCO) units are carefully and accurately rated to provide more than 90% protein recovery for proteins larger than 150,000 daltons. Pierce Concentrators are available in 7mL and 20mL sizes, which are compatible with swinging-bucket and fixed-angle rotors. These centrifugal concentrators provide efficient concentration of biological samples containing proteins (antigens, antibodies and enzymes), nucleic acids (DNA) and micro organisms (viral particles).
Highlights:
Rapid protein concentration – achieve greater than 100-fold protein concentration in less than 45 minutes
Excellent protein recovery – remove buffer and small molecules while retaining more than 90% of proteins greater than 150kDa
Convenient – two sizes designed to fit 15 and 50mL conical tubes; ideal for simple concentration of 1 to 20mL of sample
Instrument-compatible – use standard centrifuges with swinging-bucket or fixed-angle rotors; collect and recover sample without invert spinning
Versatile – the easiest way to perform sample concentration, diafiltration or buffer-exchange of biological samples
Requires:
Centrifuge equipped with a rotor for 15 or 50mL conical tubes (for 7 or 20mL Concentrators, respectively)
Recommended for:
Protein concentration with tissue culture media, antiserum or monoclonal antibody preps; Concentration of protein peaks following gel-permeation chromatography; Removal of unincorporated protein label; Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns.
