Melford Proteinase K
698,90 € HT
From Tritirachium album.
- CAS
- 39450-01-6
- Quantité
- 500 mg
- Marque
- Melford
From Tritirachium album. Serine protease for proteolytic inactivation of nucleases during isolation of DNA and RNA.
Points forts
Description
Proteinase K is a highly reactive serine protease that displays an ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is the most powerful proteinase among all proteinases characterized so far. It cleaves at the peptide bond adjacent to the carboxylic acid group of aliphatic, aromatic, or hydrophobic amino acids.
Recombinant Proteinase K is used in the isolation or preparation of high molecular weight nucleic acids. It is highly pure has a higher specific activity and is more stable at room temperature when compared to native Proteinase K.
Applications
- Recombinant Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
- Used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.
- Particularly well suited for isolating nucleic acids for amplification reactions.
- Promotes cell lysis by activating a bacterial autolytic factor.
- Used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.
Reconstitution Recommendations
For stock solution preparation, we suggest reconstituting the lyophilized powder in a buffer (20 mM Tris, 1 mM CaCl2, 2% Glycerol, pH 7.4).
Calcium is important for thermo-stability of Proteinase K and pH value is critical for high solubility.
Composition (NO ADDITIVES)
- ≥90% Protein Proteinase K, biuret test.
- Excipients: Sucrose 8%
- DNA and RNA free
- Endotoxin Free
Specifications
- Stable and active over a wide pH range of 4 to 12, Maximal Activity is in pH range of 7.5 to 12.0
- Active with SDS, urea and EDTA. The most active temperature is 65°C.
- Inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
- Purification: Nickel affinity chromatography followed by ion-exchange chromatography and dialysis.
- EC : 3.4.21.64
Caractéristiques techniques
| Marque | Melford |
|---|---|
| Réf. B-LABO | BL-RE-165707 |
| Réf. fabricant | 30018716 |
| CAS | 39450-01-6 |
| Quantité | 500 mg |
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