Sandwich ELISA Kit
The Bovine Interleukin 2 (Bov IL-2) ELISA quantitates Bov IL-2 in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov IL-2. Principle of the method The Bovine IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-105967 |
| Réf. fabricant | 17756789 |
| Numéro d’adhésion | P05016 |
| Plage de dosage | 0.273 to 200 pg/mL |
| Sensibilité du dosage | 0.2 ng/mL |
| Plage d’étalonnage | 0.2 ng/mL to 200 ng/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Other Proteins |
| Type d’échantillon | Plasma, Serum, Supernatant |
| Spécificité | Detects bovine IL-2 in. this Sandwich ELISA. It shows less than 0.2% cross-reactivity with the recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant cotton rat IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant feline IL-2. |
| Suffisant pour | 10 x 96 assays |
| Espèces cibles | Bovine |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 280822 |
| Symbole de gène(s) | IL2 |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Biotinylated Detection Antibody, Wash Buffer, Pre-coated 96 well plate, Adhesive Plate Covers, SAV-HRP, Stop Solution, Chromogen, Standard, Assay Diluent concentrate |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Immunology |
| Volume d’échantillon | Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | BOS_16374, IL-2, TCGF |
| Cible | Bovine |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
