Useful control for the western blotting application
À utiliser avec (application)
Western Blot
Tissue
Epitheloid Cervical Carcinoma
Méthode de préparation
HeLa (Human Epitheloid Carcinoma) cells, were grown to 80 to 90% confluency, then treated with 1mM pervanadate, a general inhibitor of Protein Tyrosine Phosphatases, for 15 min. at 37°C
Quantité
500 μg
Tampon de stockage
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Marque
BD
Rich in endogenously expressed proteins
Convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel
À utiliser avec (application)
Western Blot
Tissue
Epitheloid Cervical Carcinoma
Méthode de préparation
HeLa (Human Epitheloid Carcinoma) cells, were grown to 80 to 90% confluency, then treated with 1mM pervanadate, a general inhibitor of Protein Tyrosine Phosphatases, for 15 min. at 37°C
Quantité
500 μg
Tampon de stockage
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
