C32 (human amelanotic melanoma) Whole cell lysate, Denatured; Abnova
109,00 € HT
- À utiliser avec (application)
- Western Blot
- Tissue
- Skin
- Description
- Whole cell lysate (denatured)
- Tampon de lyse
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
- Méthode de préparation
- Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
- Marque
- Abnova
