Rat thymus tissue lysate, Non-denatured; Abnova
109,00 € HT
Non denatured tissue lysate of Rat thymus
- À utiliser avec (application)
- Immunoprecipitation, Western Blot
- Tissue
- Thymus
- Description
- Whole tissue lysate (non-denatured)
- Tampon de lyse
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
- Méthode de préparation
- Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
- Marque
- Abnova
