Cell transient overexpression lysate for SPHK1
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine.
Western Blotting
| Marque | Abnova |
|---|---|
| Réf. B-LABO | BL-RE-114751 |
| Réf. fabricant | 16169525 |
| À utiliser avec (application) | Western Blot |
| Lignée cellulaire | 293T |
| Numéro d’ordre du gène | NM_021972 |
| Poids moléculaire | 43.89 |
| Plage de pH | 6.8 |
| Plasmide | pCMV-SPHK1 full-length |
| Test du contrôle qualité | Transient overexpression cell Lysate was tested with Anti-SPHK1 antibody (H00008877-B01) by western blotting |
| Quantité | 100 μL |
| Tampon de stockage | 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue) |
| Espèces hôtes | Human |
| Contenu et stockage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
