Novus Biologicals™ MEF Whole Cell Lysate
301,01 € HT
- À utiliser avec (application)
- Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
- Tissue
- Bone/Cartilage
- Quantité
- 0.125 mL
- Tampon de stockage
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
- Pureté
- Multi-step
- Marque
- Novus Biologicals