"/> Novus Biologicals™ MEF Whole Cell Lysate | B-LABO France

Novus Biologicals™ MEF Whole Cell Lysate

Réf. : BL-RE-116299Réf. fabricant : 18080074 0.125 mL
301,01 € HT

À utiliser avec (application)
Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Tissue
Bone/Cartilage
Quantité
0.125 mL
Tampon de stockage
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Pureté
Multi-step
Marque
Novus Biologicals

The MEF Whole Cell Lysate is derived from mouse. It has been validated for the following applications: Western Blot, SDS-PAGE

Caractéristiques techniques

MarqueNovus Biologicals
Réf. B-LABOBL-RE-116299
Réf. fabricant18080074
À utiliser avec (application)Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
TissueBone/Cartilage
Quantité0.125 mL
Tampon de stockage1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
PuretéMulti-step
Spécificité du testMEF (Mouse Embryonic Fibroblast) whole cell extract lysate
Fraction cellulaireWhole
Contenu et stockageStore at -70°C. Avoid freeze-thaw cycles.
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