Denatured HIF1A 293T Cell Overexpression Lysate
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)
Western Blotting
| Marque | Abnova |
|---|---|
| Réf. B-LABO | BL-RE-116987 |
| Réf. fabricant | 16126111 |
| À utiliser avec (application) | Western Blot |
| Lignée cellulaire | 293T |
| Numéro d’ordre du gène | NM_181054 |
| Poids moléculaire | 82.7 |
| Plage de pH | 6.8 |
| Plasmide | pCMV-HIF1A full-length |
| Test du contrôle qualité | Transient overexpression cell Lysate was tested with Anti-HIF1A antibody (H00003091-B01) by western blotting |
| Quantité | 100 μL |
| Tampon de stockage | 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue) |
| Espèces hôtes | Human |
| Contenu et stockage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
