Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Western Blotting
| Marque | Abnova |
|---|---|
| Réf. B-LABO | BL-RE-122168 |
| Réf. fabricant | 16146534 |
| À utiliser avec (application) | Western Blot |
| Lignée cellulaire | 293T |
| Numéro d’ordre du gène | NM_007310.1 |
| Poids moléculaire | 24.4 |
| Plage de pH | 6.8 |
| Plasmide | pCMV-COMT full-length |
| Test du contrôle qualité | Transient overexpression cell Lysate was tested with Anti-COMT antibody (H00001312-B01) by western blotting |
| Quantité | 100 μL |
| Tampon de stockage | 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue) |
| Espèces hôtes | Human |
| Contenu et stockage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
