Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems,
e.g.
HaloTag™ and SNAP-tag™. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining.
To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.
Caractéristiques techniques
| Marque | Tocris |
|---|
| Réf. B-LABO | BL-RE-124699 |
|---|
| Réf. fabricant | 18794569 |
|---|
| Nom chimique ou matériau | 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9 H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt |
|---|
| CAS | 1811539-88-4 |
|---|
| Quantité | 2 mg |
|---|
| Cible | Amine-Reactive Janelia Fluor™ Dyes |
|---|
| Formule moléculaire | C<sub>34</sub>H<sub>27</sub>F<sub>4</sub>N<sub>3</sub>O<sub>6</sub> |
|---|
| Poids de la formule | Observed MW: 649.6 |
|---|
| Pureté | 95% |
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