Janelia Fluor® 635, SE is a red fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems,
e.g.
HaloTag™ and SNAP-tag™. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Also suitable for flow cytometry. Cell permeable. Excitation maximum = 635 nm; emission maximum = 652 nm; Quantum yield = 0.56; Max extinction coefficient = 167,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0524. Exhibits low nonspecific background staining.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
Caractéristiques techniques
| Marque | Tocris |
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| Réf. B-LABO | BL-RE-125838 |
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| Réf. fabricant | 18723018 |
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| Nom chimique ou matériau | 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-7-[1-(3-fluoroazetidinyl)]-9-silaanthracen-2(9 H)-ylidene]-3-fluoroazetidinium, inner salt |
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| Quantité | 2 mg |
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| Cible | Amine-Reactive Janelia Fluor™ Dyes |
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| Formule moléculaire | C<sub>33</sub>H<sub>29</sub>F<sub>2</sub>N<sub>3</sub>O<sub>6</sub>Si |
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| Poids de la formule | Observed MW: 629.69 |
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