Janelia Fluor® 646, Maleimide is a red fluorogenic fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging, super resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two color imaging with Janelia Fluor™ 549 SE (Cat. No. 6147). Cell permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54; Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-126245 |
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| Réf. fabricant | 30000821 |
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| Nom chimique ou matériau | 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1 H-pyrrol-1-yl)ethyl)carbamoyl)benzoate |
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| Quantité | 2 mg |
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| Cible | Sulfhydyl-Reactive Janelia Fluor™ Dyes |
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| Formule moléculaire | C<sub>35</sub>H<sub>34</sub>N<sub>4</sub>O<sub>5</sub>Si |
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| Poids de la formule | 618.75 |
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| Pureté | 95% |
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