These HiScreen™ columns contain Protein L resin for affinity capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.
Capacité de liaison
70 mg Human IgG/mL Resin at 6 min. Residence Time, 60 mg Human IgG/mL Resin at 4 min. Residence Time
Capacité (métrique)
4.7 mL
Diamètre (impériale) interne
0.3 in.
Diamètre (métrique) interne
7.7 mm
Débit
<300 cm/hr.
Marque
Cytiva
As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.
Redesigned protein L ligand MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to Cytiva™ Capto™ L chromatography resin. This higher capacity provides productivity advantages in research and biomanufacturing.
Caractéristiques principales
A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability
High dynamic binding capacity for efficient capture of target molecules
Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents
Provides good resolution for product-related impurities in the capture of bispecific antibodies
Convenient for method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development
Caractéristiques techniques
Marque
Cytiva
Réf. B-LABO
BL-RE-128626
Réf. fabricant
17887764
Capacité de liaison
70 mg Human IgG/mL Resin at 6 min. Residence Time, 60 mg Human IgG/mL Resin at 4 min. Residence Time
Capacité (métrique)
4.7 mL
Diamètre (impériale) interne
0.3 in.
Diamètre (métrique) interne
7.7 mm
Débit
<300 cm/hr.
Pression de fonctionnement max.
3 bar
Granulométrie
60 μm
Gamme de produits
HiScreen™ MabSelect™
Quantité
1 Column
À utiliser avec (application)
Antibody Affinity Chromatography
Cible
Antibody
Type
Pre-packed Column
Format
Chromatography Column
Hauteur du lit
100 mm
Type de colonne
Affinity
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