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STEMCELL Technologies EasySep™ Human CD4+CD127lowC… | B-LABO France
STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, with RoboSep™ Buffer and Filter Tips
Réf. : BL-RE-128840Réf. fabricant : 17197841 1 x 109 cells
1 996,50 € HT
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Type de cellule
T Cells, T Cells, CD4+, T Cells, Regulatory
À utiliser avec (application)
Cell Isolation
Quantité
1 x 109 cells
Marque
STEMCELL Technologies
Caractéristiques principales
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples
First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™
Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion
The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications
An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies
Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-128840
Réf. fabricant
17197841
Type de cellule
T Cells, T Cells, CD4+, T Cells, Regulatory
À utiliser avec (application)
Cell Isolation
Quantité
1 x 10<sup>9</sup> cells
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