Larger spin columns for simple, rapid purification of His-tagged recombinant proteins from cell lysates using Ni2+-IDA agarose resin for samples up to 20 ml and one re-use
Quantité
24 Pack
À utiliser avec (application)
Rapid purification of His-tagged recombinant Proteins from cell lysates by Metal chelate affinity chromato graphy
Type
Spin Column
Suffisant pour
2 Users
Marque
Sartorius
Vivapure maxiprep MC spin columns are centrifugal devices for rapid purification of His-tagged recombinant proteins from cell lysates by metal chelate affinity chromatography. The pre-packed spin columns incorporate Ni2+-IDA agarose resin plugs as the chromatographic matrix. The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many samples are processed in parallel. Vivapure maxiprep MC spin columns can conveniently be used in a centrifuge with swing-out rotor for standard 50 ml tubes. Binding capacity is about 10 mg His-tagged protein per unit. The low hold-up volume ensures high solute recovery with minimal loss by non-specific adsorption. Vivapure maxiprep MC spin columns can be re-used twice and are available as complete kits including buffers or as bulk packs.They are an attractive alternative to lengthy chromatographic methods such as FPLC. For processing larger sample volumes than 20 ml, e. g. from diluted cell culture supernatants, the Vivapure maxiprep spin columns can additionally be used with a sealing cap and pump collar for peristaltic pumps.
Caractéristiques principales
Spin column based kit
Devices are ready to use
Rapid purification with high yields
Parallel processing
Cost effective through re-use option
Starter kits available
Processing of larger samples with peristaltic pump
Caractéristiques techniques
Marque
Sartorius
Réf. B-LABO
BL-RE-129208
Réf. fabricant
11760865
Quantité
24 Pack
À utiliser avec (application)
Rapid purification of His-tagged recombinant Proteins from cell lysates by Metal chelate affinity chromato graphy
Type
Spin Column
Suffisant pour
2 Users
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