Thermo Scientific™ Pierce™ SILAC Protein Quantitation Kits

Ref : 10220204
652.00€ - 661.50€

Analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) using these complete reagent kits.

Description :
SILAC Protein Quantitation Kit - RPMI 1640
Formule :
Multi-component kit with heavy and light L-Lysine and RPMI 1640 Media
Contenus des kits :
L-Lysine-2HCl, 50mg, RPMI Media for SILAC, 2 x 500mL, Dialyzed FBS, 2 x 50mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6 , 50mg
Suffisant pour :
One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)
Quantité :
1 Use Kit
À utiliser avec (application) :
SILAC experiments (stable isotope labeling with amino acids in cell culture)
Brand
Thermo Scientific™

Thermo Scientific™ SILAC Protein Quantitation Kits are used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Reproducible – eliminates intra-experimental variability caused by differential sample preparation

  • Flexible – media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling

  • Compatible – label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM, RPMI-1640 or DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat and others

  • Optimized, specialized media – the Expansion DMEM and Low-Osmolarity DMEM are specifically designed and qualified for mouse embryonic stem cell propagation and SILAC protein expression profiling with mass spectrometry

  • High-quality supplements – heavy amino acids with >99% isotope purity; the Stem Cell Screened Dialyzed FBS and Serum Substitute for Mouse Embryonic Stem Cells are tested to ensure that they do not promote stem cell differentiation

Recommended for:

Quantitative analysis of relative changes in protein abundance from different cell treatments; Quantitative analysis of proteins for which there are no antibodies available; Protein expression profiling of normal vs. disease cells; Identification and quantification of hundreds to thousands of proteins in a single experiment

Description : SILAC Protein Quantitation Kit - RPMI 1640

Formule : Multi-component kit with heavy and light L-Lysine and RPMI 1640 Media

Contenus des kits : L-Lysine-2HCl, 50mg, RPMI Media for SILAC, 2 x 500mL, Dialyzed FBS, 2 x 50mL, L-Arginine-HCl, 2 x 50mg, L-Lysine-2HCl, 13C6 , 50mg

Suffisant pour : One complete SILAC experiment (stable isotope labeling with amino acids in cell culture)

Quantité : 1 Use Kit

À utiliser avec (application) : SILAC experiments (stable isotope labeling with amino acids in cell culture)

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