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Applied Biosystems™ MicroSEQ™ ID Purificatio… | B-LABO France
Applied Biosystems™ MicroSEQ™ ID Purification Combo Kit v2.0, with clean-up plates
Réf. : BL-RE-143376Réf. fabricant : 18795445
491,00 € HT
The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow.
Contenu et stockage
• 2 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C. • 2 x MicroSEQ ID Ultra Sequencing Clean-up Plate (96-well). Store at 2°C to 8°C.
Format
Plate
Type d’échantillon
Bacteria, DNA, dsDNA, Fungi
À utiliser avec (application)
Sequencing, PCR
Compatibilité à haut débit
High-throughput Compatible, Manual Protocols
Marque
Applied Biosystems
ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results. The MicroSEQ ID Ultra Sequencing Clean-up kit in 96-well plate or 8-strips format provides a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions. The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer.
Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Ultra Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure below). Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result.
ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits:
MicroSEQ ID Ultra Sequencing Clean-up Plates can be used downstream of the following sequencing kits:
Caractéristiques principales
MicroSEQ 500 16S rDNA PCR Kit
MicroSEQ Full Gene 16S rDNA PCR Kit
MicroSEQD2 rDNA Fungal PCR Kit
MicroSEQ 500 16S rDNA Sequencing Kit
MicroSEQ Full Gene 16S rDNA Sequencing Kit
MicroSEQ D2 rDNA Fungal Sequencing Kit
Caractéristiques techniques
Marque
Applied Biosystems
Réf. B-LABO
BL-RE-143376
Réf. fabricant
18795445
Contenu et stockage
• 2 x ExoSAP-IT Express Reagent (100 reactions). Store at -5°C to -30°C.<br>• 2 x MicroSEQ ID Ultra Sequencing Clean-up Plate (96-well). Store at 2°C to 8°C.
Format
Plate
Type d’échantillon
Bacteria, DNA, dsDNA, Fungi
À utiliser avec (application)
Sequencing, PCR
Compatibilité à haut débit
High-throughput Compatible, Manual Protocols
Nbre de réactions
100 Reactions
Gamme de produits
MicroSEQ™
Conditions d’expédition
Approved for shipment on Wet or Dry Ice
Cible
Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)
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