"/> Invitrogen™ Kit ELISA TNF alpha de rats | B-LABO France

Invitrogen™ Kit ELISA TNF alpha de rats

Réf. : BL-RE-144298Réf. fabricant : 10741585 1 Plate Kit
539,70 € HT

Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.

Plage de dosage
31 to 2500pg/mL
Sensibilité du dosage
<15pg/mL
Description
Rat TNF alpha ELISA Kit
Format du kit d’immunoessai
Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Marque
Invitrogen

Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum, plasma (EDTA, heparin) and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.

These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Caractéristiques principales

  • Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31 to 2500pg/mL
  • Sensitivity: < 15pg/mL
  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-144298
Réf. fabricant10741585
Plage de dosage31 to 2500pg/mL
Sensibilité du dosage<15pg/mL
Format du kit d’immunoessaiColorimetric ELISA (TMB substrate) with Plates
Type d’échantillonCell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
SpécificitéNo significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
Suffisant pour96 microplate wells
Contenus des kitsStandard Diluent, 25mL, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Pre-Treatment Buffer, 6mL, Lyophilized Recombinant Rat TNF alpha Standard, 2 vials, Biotinylated Antibody Reagent, 6mL, Streptavidin-HRP Reagent, 14mL, Anti-Rat TNF alpha Precoated 96-well Strip Plate, 1 ea, 30X Wash Buffer, 50mL
MesureAbsorbance at 450nm minus absorbance at 550nm
PrécisionIntra-assay CV <10%; inter-assay CV <10%
Quantité1 Plate Kit
Volume d’échantillon50 μL
CibleNatural and recombinant rat TNFa (rat tumor necrosis factor alpha)
Durée totale du dosage3 hr.
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