LNCaP clone FGC (human prostate carcinoma) Whole cell lysate, Denatured; Abnova

Réf. : BL-RE-146492Réf. fabricant : 16079495 200 μg

109,00 € HT

À utiliser avec (application): Western Blot
Tissue: Prostate
Description: Whole cell lysate (denatured)
Tampon de lyse: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Méthode de préparation: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Marque: Abnova

Caractéristiques principales

Whole cell lysate (denatured)

Tissue: prostate

Host: Human

Preparation: homogenization in ice-cold modified RIPA Lysis

Lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, 1mM PMSF

Storage buffer: in ready-to-use 1X sample buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% bromophenol blue)

Caractéristiques techniques

Marque	Abnova
Réf. B-LABO	BL-RE-146492
Réf. fabricant	16079495
À utiliser avec (application)	Western Blot
Tissue	Prostate
Tampon de lyse	Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Méthode de préparation	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Test du contrôle qualité	12.5% SDS-PAGE Stained with Coomassie Blue.
Quantité	200 μg
Tampon de stockage	In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Espèces hôtes	Human
Concentration	3 mg/mL
Contenu et stockage	Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Une question, une configuration particulière ?
Contactez-nous pour un devis.

Demander un devis 03 65 67 15 79

LNCaP clone FGC (human prostate carcinoma) Whole cell lysate, Denatured; Abnova

Caractéristiques principales

Caractéristiques techniques

Produits similaires

Lysat de surexpression TUBE1 en cellules 293T (dénaturé), Abnova

Lysat de surexpression PER1 en cellules 293T (dénaturé), Abnova

Lysat de surexpression SORBS3 en cellules 293T (dénaturé), Abnova

Lysat de surexpression RAB23 en cellules 293T 1 (dénaturé), Abnova