Points forts
Key information
PA Janelia Fluor™ 549, SE is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.
Application
Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.
Properties and Photophysical Data
Non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Can be multiplexed with PA Janelia Fluor™ 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively. Please see the protocol for further information on protein/antibody labeling and conjugation.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-151313 |
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| Réf. fabricant | 18024972 |
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| Nom chimique ou matériau | 2,5-Dioxo-1-pyrrolidinyl 3,6-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9-[9H]xanthene]-6-carboxylate |
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| CAS | 1811539-42-0 |
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| Quantité | 300 μg |
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| Cible | Photoactivatable Dyes |
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| Formule moléculaire | C<sub>32</sub>H<sub>25</sub>N<sub>5</sub>O<sub>6</sub> |
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| Pureté | 90% |
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