Viral transduction enhancer
Polybrene is a cationic polymer that improves lentiviral and adenoviral transduction efficiency in mammalian cells
in vitro
. It also enhances DNA transfection in many types of cells. It has a neutralizing effect on negatively charged DNA and viral particles which facilitates their attachment and entry into host cells. Polybrene is suitable for use with CRISPR/Cas9 protocols.
Polybrene is a lyophilized solid and can be prepared as a stock solution by adding 1 mL of sterile ultra-pure water directly to the vial (final stock concentration 10 mg/mL).
| Marque | Tocris Bioscience |
|---|---|
| Réf. B-LABO | BL-RE-151600 |
| Réf. fabricant | 18717326 |
| Nom chimique ou matériau | 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide |
| CAS | 28728-55-4 |
| Quantité | 10 mg |
| Cible | Viral Transduction Enhancers |
| Pureté | 0.95 |
