Thermo Scientific™ Pfu DNA Polymerase

Ref : 10180690
71.50€ - 317.00€

Optimize PCR applications requiring high fidelity with this highly thermostable DNA polymerase.

Description :
Pfu DNA Polymerase
Quantité :
500U
À utiliser avec (application) :
High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis
Thermostabilité :
remains 95% active after 2 hours incubation at 95°C
Comprend :
2 x 1.25mL 10X Pfu Buffer with (NH4)2SO2, 2 x 1.25mL 10X Pfu Buffer with MgSO4 and 2 x 1.25mL 25mM MgSO4
Brand
Thermo Scientific™

Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction. Pfu DNA Polymerase also exhibits 3’→5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’→3’ exonuclease activity.

Highlights

  • Eight times more accurate than Taq DNA polymerase
  • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
  • Generates blunt-end PCR products
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
  • Increased PCR yields with native Pfu DNA Polymerase

Description : Pfu DNA Polymerase

Quantité : 500U

À utiliser avec (application) : High-fidelity PCR, Standard PCR, Cloning, RT-PCR, Site-directed Mutagenesis

Thermostabilité : remains 95% active after 2 hours incubation at 95°C

Comprend : 2 x 1.25mL 10X Pfu Buffer with (NH4)2SO2, 2 x 1.25mL 10X Pfu Buffer with MgSO4 and 2 x 1.25mL 25mM MgSO4

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