This kit contains a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Caractéristiques principales
Antibody specific for mouse/rat HGF has been pre-coated onto the microwells
Mouse/rat HGF protein in samples is captured by the coated antibody after incubation
Following extensive washing, another antibody of biotinylated specific for mouse/rat HGF is added to detect the captured mouse/rat HGF protein
For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
