"/> Invitrogen™ Kit ELISA IL-1 alpha humains | B-LABO France

Invitrogen™ Kit ELISA IL-1 alpha humains

Réf. : BL-RE-155531Réf. fabricant : 10066023 1 Plate Kit
548,10 € HT

Measure levels of human interleukin-1 alpha (IL-1a) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Plage de dosage
10.2 to 400pg/mL
Sensibilité du dosage
<2pg/mL
Description
Human IL-1 alpha ELISA Kit
Format du kit d’immunoessai
Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
Marque
Invitrogen

Assay IL-1a (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-1 alpha ELISA Kits.

These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Caractéristiques principales

  • Target: Natural and recombinant human interleukin-1 alpha (IL-1α)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 10.2 to 400pg/mL
  • Sensitivity: < 2pg/mL
  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-155531
Réf. fabricant10066023
Plage de dosage10.2 to 400pg/mL
Sensibilité du dosage<2pg/mL
Format du kit d’immunoessaiColorimetric ELISA (TMB substrate) with Plates
Type d’échantillonCell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
SpécificitéNo significant cross-reaction with human IFNa, IFNg, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, or mouse IL-1a. Human IL-1ra does not interfere with this ELISA
Suffisant pour96 microplate wells
Contenus des kitsStop Solution, 13mL, Streptavidin-HRP Concentrate, 75µL, Standard Diluent, 12mL, contains 0.01% thimerosal, Biotinylated Antibody Reagent, 8mL, TMB Substrate Solution, 13mL, Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 1 ea, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human IL-1 alpha Standard, 2 vials, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL
MesureAbsorbance at 450nm minus absorbance at 550nm
PrécisionIntra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Quantité1 Plate Kit
Volume d’échantillon50 μL
CibleNatural and recombinant human interleukin-1 alpha (IL-1a)
Durée totale du dosage3 hr.

Documentation

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