Points forts
Key information
Janelia Fluor™ 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.
Application
Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data
Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169; lactone-zwitterion equilibrium constant (KL-Z) = 3.5. Please see the product protocol for further information and a guide to protein/antibody labeling.
Caractéristiques techniques
| Marque | Tocris Bioscience |
| Réf. B-LABO | BL-RE-158171 |
| Réf. fabricant | 18095312 |
| Nom chimique ou matériau | 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate |
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| Quantité | 2 mg |
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| Cible | Sulfhydyl-Reactive Janelia Fluor Dyes |
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| Formule moléculaire | C<sub>33</sub>H<sub>28</sub>N<sub>4</sub>O<sub>6</sub>.C<sub>2</sub>HF<sub>3</sub>O<sub>2</sub> |
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| Pureté | 95% |
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