This gene, CYP3A4, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4.
Western Blotting
| Marque | Abnova |
|---|---|
| Réf. B-LABO | BL-RE-163027 |
| Réf. fabricant | 16143841 |
| Lignée cellulaire | 293T |
| À utiliser avec (application) | Western Blot |
| Numéro d’ordre du gène | BC101631.1 |
| Poids moléculaire | 57.3 |
| Plage de pH | 6.8 |
| Plasmide | pCMV-CYP3A4 full-length |
| Test du contrôle qualité | Transient overexpression cell Lysate was tested with Anti-CYP3A4 antibody (H00001576-B01) by western blotting |
| Quantité | 100 μL |
| Tampon de stockage | 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue) |
| Espèces hôtes | Human |
| Contenu et stockage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
