Spontaneously Blinking Janelia Fluor™ Dyes allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers. These spontaneously blinking dyes harness Janelia Fluor™ technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.
Key Information: Janelia Fluor™ 646b, NHS ester is a spontaneously blinking fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.
Used for: suitable for DNA labeling using amine-terminated oligonucleotides in RNA-FISH experiments.
Application: suitable for super resolution microscopy (SRM) including single-molecule localization microscopy (SMLM).
Caractéristiques techniques
| Marque | Tocris Bioscience |
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| Réf. B-LABO | BL-RE-165682 |
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| Réf. fabricant | 30058514 |
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| Nom chimique ou matériau | 2,5-Dioxopyrrolidin-1-yl 3,7-di(azetidin-1-yl)-5,5-dimethyl-3'H,5H-spiro[dibenzo[b,e]siline-10,1'-isobenzofuran]-6'-carboxylate |
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| Quantité | 200 μg |
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| Cible | Amine-Reactive Janelia Fluor Dyes |
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| Formule moléculaire | C<sub>33</sub>H<sub>33</sub>N<sub>3</sub>O<sub>5</sub>Si |
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| Pureté | 0.9 |
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