Hygromycin B is an antibiotic that is derived from Streptomyces hygroscopicus and has broad-spectrum activity against a wide range of bacteria, yeast, and higher eukaryotes.
In molecular biology and genetic engineering, Hygromycin B is widely employed as a selective agent. It is commonly used in the selection of cells or organisms that have been successfully transformed with a hygromycin resistance gene. The resistance gene encodes an enzyme called hygromycin phosphotransferase, which phosphorylates and inactivates Hygromycin B, allowing only the transformed cells to survive and propagate.
Hygromycin B is also used in cell culture applications. It is effective in preventing the growth of bacterial and fungal contaminants in cell cultures, ensuring the purity of cell lines. It is often added to the growth media at appropriate concentrations to selectively eliminate non-transformed or contaminated cells.
| Marque | Melford |
|---|---|
| Réf. B-LABO | BL-RE-165744 |
| Réf. fabricant | 30018330 |
| Nom chimique ou matériau | Hygromycin B, Powder |
| CAS | 31282-04-9 |
| Formule moléculaire | C20H37N3O13 |
| Quantité | 1 g |
