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Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) labeled proteins
Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor™ 585 is cell permeable.
Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (70%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor™ 585; excitation and emission maxima (λ) are 590 nm and 620 nm, respectively; quantum yield = 0.52; extinction coefficient = 123,000 M-1cm-1 (in 4% TFA).
It is recommended to use a working concentration of 10 μM in live cell imaging application.
