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Thermo Scientific™ CaptureSelect™ KappaXP RoboColumn™ Pre-packed columns

Ref: 30274999

530.00

Thermo Scientific™ CaptureSelect™ RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

Contenu et stockage:

Store in refrigerator (2–8°C).<br><br>

Conditions d’expédition:

Wet Ice

Granulométrie:

65 ±10 μm

Gamme de produits:

CaptureSelect™

Quantité:

200 μL

Type:

Colonne

Brand

Thermo Scientific™

CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect KappaXP matrix recognizes the kappa light-chain of human antibodies, enabling purification of all Kappa containing Ig's, Fab, and Fab2 fragments directly from complex sources and feed streams.

Features of this affinity matrix include

Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
Mild elution for Fab fragments and antibodies
Human-specific, no binding to bovine antibodies
Excellent scalability
Excellent pH stability up to 100 mM NaOH (limited exposure)
Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

Column size: 200 μL, 1 strip, 8 columns (8-row)
Column dimension: 5-mm diameter, 10-mm bed height
Matrix: agarose based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide coupling
Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height, and residence time
Elution conditions: 20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl₂, pH6
Formulation buffer: 20% (v/v) ethanol
Max operating pressure: 2 bar
Flow rate: 16–150 cm/hr