This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq]
Caractéristiques techniques
| Marque | Abnova |
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| Réf. B-LABO | BL-RE-056552 |
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| Réf. fabricant | 16202270 |
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| À utiliser avec (application) | SDS-PAGE |
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| Formule | Lyophilized from 1 ml of 10mM sodium deoxycholate, 50mM NaCl, 5 0mM Na |
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| Identification génétique (Entrez) | 338 |
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| Nom | APOB (Human) Native Protein |
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| Méthode de préparation | Purified from low density lipoproteins (LDL) isolated by density gradient ultracentrifugation (1.03 - 1.05 g/mL density range) and by gel filtration chromatography in 10 mM sodium deoxycholate, 50 mM NaCl, 50 mM Na<sub>2</sub>CO<sub>3</sub>, pH 10.1. |
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| Méthode de purification | Density gradient ultracentrifugation and gel filtration chromatography |
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| Quantité | 500 ug |
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| Conditions de stockage | Store at -20°C or lower on dry atmosphere. After reconstitution, store at 2-8°C no longer than 4 weeks. Reconstitution: Dissolve lyophilized Apo B in 100 mL to 1 mL of distilled or deionized water. If dissolving in solvent we recommend using 10 mM sodium deoxycholate, 50 mM NaCl, 50 mM Na<sub>2</sub>CO<sub>3</sub>, pH 10 to keep the sodium deoxycholate at an acceptable level instead of water. |
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| État réglementaire | RUO |
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| Alias de gène | FLDB |
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| Nom usuel | APOB |
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| Symbole de gène(s) | APOB |
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| Espèces | Human |
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| Marqueur de protéine | None |
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| Système d’expression | Purified from low density lipoproteins (LDL) isolated by density gradient ultracentrifugation (1.03 - 1.05 g/mL density range) and by gel filtration chromatography in 10 mM sodium deoxycholate, 50 mM NaCl, 50 mM Na<sub>2</sub>CO<sub>3</sub>, pH 10.1. |
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| Forme | Lyophilized |
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| Pureté ou qualité | ≥95% by SDS-PAGE |
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