Rapidly detect E. coli O157:H7, stx, eae, in food samples in as little as 10 hours with highly-specific, simple to use real-time PCR ThermoScientific Suretect Escherichia coli O157:H7 and STEC Screening PCR Assay.
Gamme de produits
Suretect
Classe d’organisme cible
Rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145)
À utiliser avec (application)
Food & Beverage
Marque
Thermo Scientific
Rapidly detect multiple genetic targets for Shiga-toxin producing Escherichia coli O157:H7 and other STEC in food and environmental samples with the Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC PCR workflow. The workflow has been granted AOAC Performance Tested MethodsSM Certification from the AOAC Research Institute and NF VALIDATION™ from AFNOR (ISO 16140-2:2016).
The assay has been extensively tested on a range of food matrices from food manufacturing environments, including raw meat (excluding poultry), vegetables, fruits, juices, and raw dairy samples and enables users to reliably detect and differentiate Shiga-toxin producing Escherichia coli O157:H7 and other STEC in as little as 10 hours.
The SureTect PCR System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.
The Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay is part of a complete workflow for the rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145) from the tested samples.
Points forts
Simple
Easy-to-use with single enrichment step and pre-dispensed reagents
Target-specific colored assay plastics
Clearly oriented lysis and assay trays
Color indicator in lysis reagent reducing possibility for human error
Fast and Flexible
Option to automate the lysis and PCR sample preparation
Optimized workflow delivers on-screen results in as little as 10 hours
Maximum flexibility for operational efficiency
Run all assays simultaneously in the same run
Test up to 96 samples in each run
Choice of pierceable seals or caps
Smart
Intuitive, user-friendly software delivers reliable results, with no subjective interpretation
Actionable data supports fast decisions in a complex product supply chain
IPC in every assay for complete confidence in results
Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay includes
Enriched samples are pipetted into pre-filled lysis tubes, along with Proteinase K, before incubation, to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Escherichia coli O157:H7 and STEC Screening PCR tubes to re-hydrate the pre-loaded PCR pellet which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal positive control (IPC). The PCR tubes are sealed, then loaded into the Applied Biosystems™ QuantStudio™ 5 Food Safety Real Time PCR Instrument with RapidFinder™ Analysis Software v3.0 or later. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required. The internal amplification control confirms correct assay conditions have occurred.
Not all products are available for sale in all territories. Please inquire.
Lysis Reagent 1 Tubes
Proteinase K
Escherichia coli O157:H7 and STEC Screening PCR Tubes
Thermo Scientific 96-Well Pierceable Seals
PCR Caps
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-EQ-071203
Réf. fabricant
17819142
Gamme de produits
Suretect
Classe d’organisme cible
Rapid detection of Escherichia coli O157:H7, stx, eae, and the Escherichia coli STEC serotypes (O26, O45, O103, O111, O121, O145)
À utiliser avec (application)
Food & Beverage
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