Functional assays: Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. 3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures. Gelling assay: BME gels in less than 30 minutes at 37degreesC, and maintains the gelled form in culture medium for a minimum of 14 days at 37degreesC.
- Contenu et stockage
- Product is stable for a minimum of 3 months from date of shipment when stored at -20° C in a manual defrost freezer. For optimal stability, store at -80° C. Avoid freeze-thaw cycles.
- Concentration
- 14 to 16 mg/mL
- Niveau d’endotoxines
- ≤8EU/mL
- À utiliser avec (application)
- Bioactivity
- Quantité
- 2 x 5 mL
- Source
- Murine Engelbreth-Holm-Swarm (EHS) tumor.
