Methylcellulose-based medium with recombinant cytokines for rapid hematopoietic colony assays of human cells, IVD
Type de cellule
Hematopoietic Stem and Progenitor Cells
À utiliser avec (application)
Cell Culture, Colony Assay, Functional Assay
Quantité
100 mL
Marque
STEMCELL Technologies
MethoCult™ Express is intended for use in hematopoietic colony-forming unit (CFU) assays of human cord blood (CB) samples, after a minimum culture period of 7 days
It is recommended for use with red blood cell-depleted CB samples, whole CB samples that have been cryopreserved and thawed, and CB mononuclear cells
MethoCult™ Express is optimized for the growth and enumeration of human hematopoietic progenitor cells, after a much shorter period than the 14 -16 days of standard CFU assays
In MethoCult™ Express, colonies containing at least 20 cells can be counted as early as after 7 days of culture
If MethoCult™ Express cultures are maintained for 14 - 16 days, colonies derived from erythroid progenitor cells (BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, and CFU-M), and multi-potential granulocyte, erythroid, macrophage, and megakaryocyte progenitor cells (CFU-GEMM) can be counted
