Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
À utiliser avec (application)
Cloning of blunt-end or 3'-dA tailed PCR products up to 10kb; Cloning of DNA fragments generated by restriction enzymes; Sequencing of cloned DNA; In vitro and in vivo transcription of cloned inserts from the T7 promoter
Nbre de réactions
20
Type de produit
CloneJET™ PCR Cloning Kit
Marque
Thermo Scientific
CloneJET PCR Cloning Kit is an advanced positive selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any other blunt or sticky-end DNA fragment can be cloned. It is ideal for phosphorylated or non-phosphorylated DNA fragments. Ligation into the included positive selection vector takes only 5 minutes, yielding more than 99% recombinant clones. Blunt-ended PCR products generated with a proofreading enzyme are ligated directly into the cloning vector.
PCR products generated either with non-proofreading DNA polymerases or mixtures of DNA polymerases are blunted prior to ligation in 5 minutes with the thermostable DNA Blunting Enzyme provided with the kit. All common laboratory E. coli strains can be directly transformed with the ligation product.
Highlights
The CloneJET PCR Cloning Kit contains a novel, ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularized pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.
Caractéristiques principales
Fast - PCR cloning in only 5 minutes
Highest efficiency - > 99% of positive clones
No cloning background - positive selection vector
Versatile - ideal for blunt-end or sticky-end cloning
Economical - no expensive blue/white screening
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-087426
Réf. fabricant
15727526
À utiliser avec (application)
Cloning of blunt-end or 3'-dA tailed PCR products up to 10kb; Cloning of DNA fragments generated by restriction enzymes; Sequencing of cloned DNA; In vitro and in vivo transcription of cloned inserts from the T7 promoter
