A non-integrating, self-replicating RNA reprogramming vector for generating iPS cells
Type de cellule
Pluripotent Stem Cells
À utiliser avec (application)
Cell Culture; Reprogramming
Quantité
12 μg
Marque
STEMCELL Technologies
Caractéristiques principales
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1)
This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2)
When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4)
ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6)
Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media (mTeSR™1, TeSR™2, or TeSR™-E8™) and further differentiated into cells of all three germ layers (Figure 7)
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-088274
Réf. fabricant
17151036
Type de cellule
Pluripotent Stem Cells
À utiliser avec (application)
Cell Culture; Reprogramming
Quantité
12 μg
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