"/> MP Biomedicals™ SPINeasy™ Plasmid Midiprep K… | B-LABO France

MP Biomedicals™ SPINeasy™ Plasmid Midiprep Kit

Réf. : BL-RE-089072Réf. fabricant : 17894126 25 Preps
385,00 € HT

Spin column technology for fast and efficient purification of both high and low copy plasmids from bacterial cultures. High-quality plasmid DNA is free of gDNA contamination following a modified alkaline lysis method.

Contenu et stockage
Store at Room Temperature, Dry Environment
Isolation Technology
Spin Column
Type d’échantillon
25 to 50 mL Bacterial (E. coli) Cultures
Comprend
60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
Quantité
25 Preps
Marque
MP Biomedicals

Caractéristiques principales

  • SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 mL of transformed bacterial cultures.
  • Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA.
  • Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the supernatant is adsorbed on a silica membrane, washed, and eluted in a convenient process using spin column technology.
  • Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology applications, including cloning, in-vitro transcription, PCR, and restriction digestion.

Caractéristiques techniques

MarqueMP Biomedicals
Réf. B-LABOBL-RE-089072
Réf. fabricant17894126
Contenu et stockageStore at Room Temperature, Dry Environment
Isolation TechnologySpin Column
Type d’échantillon25 to 50 mL Bacterial (E. coli) Cultures
Comprend60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
Quantité25 Preps
Une question, une configuration particulière ?
Contactez-nous pour un devis.