Use in ICC/IF Reported in Scientific Literature (PMID:34082772) For Amplification, Transform a Suitable E. Coli Host Such as HB101 or DH-5 Alpha and Plate on LB Plates Containing 50-ug/mL Ampicillin. For Plasmid DNA Preparation Start Culture From a Single Colony in LB Medium Containing 100 ug/mL Ampicillin. Use in Retroviral Production Reported in Scientific Literature (PMID: 24311986).
Marque
Novus Biologicals
The pCL-Ampho packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of 1 infectious particle/cell/day. Retrovirus tropism is determined at three levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. The plasmid DNA can be amplified by transforming bacteria and growing them on LB/ampicillin (100 ug/ml) media.
Points forts
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Caractéristiques principales
Species: Human
Specificity: Ectotropic (usually) (MoMuLV) Mouse and rat cells only (not human), Amphotropic (from 4070A MuLV) Most mammalian cells (but not hamster) 10A1 (MuLV) Most mammalian cells (including hamster)
Buffer: 10μg in 20μL sterile water
Caractéristiques techniques
Marque
Novus Biologicals
Réf. B-LABO
BL-RE-090308
Réf. fabricant
18746853
Tampon de stockage
10µg in 20µL sterile water
Contenu et stockage
Store at -20°C. Avoid freeze-thaw cycles.
Quantité
10 μg
Vecteur
pCL-Ampho Retrovirus Packaging Vector
À utiliser avec (application)
Use in ICC/IF Reported in Scientific Literature (PMID:34082772) For Amplification, Transform a Suitable E. Coli Host Such as HB101 or DH-5 Alpha and Plate on LB Plates Containing 50-ug/mL Ampicillin. For Plasmid DNA Preparation Start Culture From a Single Colony in LB Medium Containing 100 ug/mL Ampicillin. Use in Retroviral Production Reported in Scientific Literature (PMID: 24311986).
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