Pour la détection quantitative des concentrations de MIP-1bêta dans les surnageants, le sérum et le plasma de culture cellulaire.
The Human Macrophage Inflammatory Protein-1beta (CCL4)(Hu MIP-1β) ELISA quantitates Hu MIP-1β in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu MIP-1β. Principle of the method The Human MIP-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Plaque(s) pré-enduite(s)
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-096417 |
| Réf. fabricant | 15561527 |
| Numéro d’adhésion | P13236 |
| Plage de dosage | 7,8-500 pg/ml |
| Sensibilité du dosage | 3,0 pg/ml |
| Conjugué | Biotine |
| Type de produit | Kit ELISA |
| Type d’échantillon | Sérum, Surnageant, Supernatant |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Identification génétique (Entrez) | 6351 |
| Symbole de gène(s) | CCL4 |
| Coefficient de variation inter-dosage | 4.9% |
| Coefficient de variation intra-dosage | 7.2% |
| Contenus des kits | Couvercles de plaques adhésives, Tampon de lyse 10x, Anticorps de détection biotinylé, Anticorps de détection biotinylé, lyophilisé, Microplaque à 96 puits préenduite, Solution d’arrêt, Diluant d’échantillon, Solution de substrat, Solution de substrat, Concentrat de tampon de lavage |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 128 tests |
| État réglementaire | RUO |
| Volume d’échantillon | Plasma, 50 μl ; Sérum, 50 μl ; Surnageant, 50 μl |
| Conditions de stockage | -20°C |
| Synonyme | CCL4, LAG-1, cytokine A4 inductible de petite taille, SCYA4 |
| Cible | Humaine |
| Heure du test | 20 min |
| Durée totale du dosage | 3 h 10 min |
