"/> Invitrogen™ Kit ELISA MCP-1 de souriss | B-LABO France

Invitrogen™ Kit ELISA MCP-1 de souriss

Réf. : BL-RE-097370Réf. fabricant : 10107783 1 Plate Kit
548,10 € HT

Measure levels of mouse monocyte chemoattractant protein-1 (MCP1) in mouse serum, plasma and cell culture supernatant samples using 96-well plates.

Plage de dosage
16 to 1000pg/mL
Sensibilité du dosage
<4pg/mL
Description
Mouse MCP-1 ELISA Kit
Format du kit d’immunoessai
Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Marque
Invitrogen

Assay MCP-1 (mouse monocyte chemoattractant protein-1) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific™ Mouse MCP-1 ELISA Kits.

These mouse MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.

Caractéristiques principales

  • Target: Natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 16 to 1000pg/mL
  • Sensitivity: < 4pg/mL
  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1α, RANTES or TNFα, or human or rat MCP-1

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-097370
Réf. fabricant10107783
Plage de dosage16 to 1000pg/mL
Sensibilité du dosage<4pg/mL
Format du kit d’immunoessaiColorimetric ELISA (TMB substrate) with Plates
Type d’échantillonCell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
SpécificitéNo significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1a, RANTES or TNFa, or human or rat MCP-1
Suffisant pour96 microplate wells
Contenus des kitsTMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75µL, Stop Solution, 13mL, 30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Mouse MCP-1 Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 12mL, HRP Dilution Buffer, 13mL, Lyophilized Recombinant Mouse MCP-1 Standard, 2 vials, Sample Diluent, 12mL
MesureAbsorbance at 450nm minus absorbance at 550nm
PrécisionIntra-assay CV <10%; inter-assay CV <10%
Quantité1 Plate Kit
Volume d’échantillon50 μL
CibleNatural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)
Durée totale du dosage3 hr.
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