This shuttle vector contains the complete ORF for the gene of interest, along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector.
Expression
PFKFB4
Numéro d’adhésion
NP_004558
Concentration
10 μg at 0.2 μg/μL
Formule
cDNA is provided in 10 mM Tris-Cl, pH 8.5
Type d’insert
1423 bp
Marque
R&D Systems
Caractéristiques principales
PRKRB4 is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6- bisphosphate (F2,6BP).
It is highly expressed in cancer cells and is induced by hypoxia.
PRKRB4 is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them.
Alternatively spliced transcripts encoding different proteins have been described.
The insert is sequence verified and the entire plasmid DNA and insert translated sequences are provided.
For reasearch use only. Not for use in humans.
Caractéristiques techniques
Marque
R&D Systems
Réf. B-LABO
BL-RE-100072
Réf. fabricant
18790379
Expression
PFKFB4
Numéro d’adhésion
NP_004558
Concentration
10 μg at 0.2 μg/μL
Formule
cDNA is provided in 10 mM Tris-Cl, pH 8.5
Type d’insert
1423 bp
Identification génétique (Entrez)
5210
Alias de gène
6 PF-2-K/Fru-2,6-P2ase 4, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4,6 PF-2-K/Fru-2,6-P2ase 4,6 PF-2-K/Fru-2,6-P2ase testis-type isozyme, bifunctional enzyme with kinase and biphosphatase activities, PFK/FBPase 4
Contenu et stockage
Store the unopened product at -20°C to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Espèces
Human
Quantité
10 μg
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