The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands.
À utiliser avec (application)
Tests de liaison compétitifs, dosage du récepteur nucléaire
Quantité
400 dosages de 20 μl
Contenu et stockage
• Progesterone Receptor Ligand Binding Domain Recombinant Protein, 1 tube (store at -68°C to -85°C) • Fluormone PL Red Tracer, 1 tube (-5°C to -30°C) • PR Red Screening Buffer, 2 bottles (-5°C to -30°C) • DTT, 1 tube (-5°C to -30°C)
Gamme de produits
PolarScreen™
Ligand
PR
Marque
Thermo Scientific
This kit produces a red color read-out; a 'green' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.
The PolarScreen Progesterone Receptor Competitor Assay is:
Points forts
How the Assay Works
An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently-tagged progesterone ligand (Fluormone PL Red) in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a PL Red/PR-LBD(GST) complex resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).
Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
Caractéristiques principales
Complete & ready-to-use out of the box—all reagents included, just add your test compounds
High throughput compatible—fluorescence polarization assays in a 384-well plate
Convenient—components available in bulk sizes for larger screening needs
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-102356
Réf. fabricant
15667858
À utiliser avec (application)
Tests de liaison compétitifs, dosage du récepteur nucléaire
Quantité
400 dosages de 20 μl
Contenu et stockage
• Progesterone Receptor Ligand Binding Domain Recombinant Protein, 1 tube (store at -68°C to -85°C)<br>• Fluormone PL Red Tracer, 1 tube (-5°C to -30°C)<br>• PR Red Screening Buffer, 2 bottles (-5°C to -30°C)<br>• DTT, 1 tube (-5°C to -30°C)
Gamme de produits
PolarScreen™
Ligand
PR
Type de produit
Kit d’essai
No. of Assays
400 x 20 μL Assays
Une question, une configuration particulière ? Contactez-nous pour un devis.
