The PolarScreen™ Vitamin D Receptor Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential vitamin D receptor (VDR) ligands.
À utiliser avec (application)
Tests de liaison compétitifs, dosage du récepteur nucléaire
Quantité
800 dosages de 20 μl
Contenu et stockage
• VDR Recombinant Human Protein, 1 tube (store at -68°C to -85°C) • Fluormone VDR Red Tracer, 1 tube (-5°C to -30°C) • VDR Red Screening Buffer, 3 bottles (-5°C to -30°C) • DTT, 1 tube (-5°C to -30°C)
Gamme de produits
PolarScreen™
Ligand
VDR
Marque
Thermo Scientific
This kit produces a red color read-out and is suitable to determine the IC50s of competitive compounds. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.
The PolarScreen Vitamine D Receptor Competitor Assay is:
Points forts
How the Assay Works
VDR is added to a fluorescent VDR ligand (Fluormone VDR Red) to form a receptor/tracer complex, resulting in a high polarization value. This complex is then added to individual test compounds in microwell plates. Competitors displace the tracer from the complex, causing the fluorescent ligand to tumble more rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex; therefore, the polarization value will remain high. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for VDR (see figure).
Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
Caractéristiques principales
Complete & ready-to-use out of the box—all reagents included, just add your test compounds
High throughput compatible—fluorescence polarization assays in a 384-well plate
Convenient—components available in bulk sizes for larger screening needs
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-102364
Réf. fabricant
15677858
À utiliser avec (application)
Tests de liaison compétitifs, dosage du récepteur nucléaire
Quantité
800 dosages de 20 μl
Contenu et stockage
• VDR Recombinant Human Protein, 1 tube (store at -68°C to -85°C)<br>• Fluormone VDR Red Tracer, 1 tube (-5°C to -30°C)<br>• VDR Red Screening Buffer, 3 bottles (-5°C to -30°C)<br>• DTT, 1 tube (-5°C to -30°C)
Gamme de produits
PolarScreen™
Ligand
VDR
Type de produit
Kit de dosage compétitif du récepteur de la vitamine D
No. of Assays
800 x 20 μL Assays
Une question, une configuration particulière ? Contactez-nous pour un devis.
