The Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) enables optimal image-based analysis of two key markers of human pluripotent stem cells (PSCs): OCT4 and SSEA4.
Description
Kit d’immunocytochimie de cellules souches pluripotentes (OCT4, SSEA4), 40, tests, liquide, 2 couleur, Alexa Fluor™ 488, Alexa Fluor™ 555, étiquette Alexa Fluor ™ 594, utilisée dans l’imagerie cellulaire, analyse de cellules souches, température de conservation de –20°C, glace sèche
Type de produit
Kit d’immunocytochimie
Quantité
40 Tests
À utiliser avec (application)
Analyse cellulaire, Imagerie cellulaire, Recherche sur les cellules souches, Analyse de cellules souches, Marquage et détection des cellules souches, Immunocytochimie (ICC)
Conjugué
2 couleurs, Alexa Fluor 488, Alexa Fluor 555
Marque
Invitrogen
With the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4), you can:
Points forts
Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 is a critical nuclear marker, while SSEA4 is an important cell surface marker.
Get More Information Per Sample
The antibodies in the kit have been designed to allow for the specific and simultaneous assessment of OCT4 and SSEA4 along with nuclear DNA staining to help save time and precious sample.
Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.
Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.
Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.
What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)
Caractéristiques principales
Confirm expression of key pluripotent stem cell markers with highly specific antibodies
Get more information per sample by measuring more than one marker at a time
Generate beautiful multiplexed images with minimal effort
Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
Process samples with confidence using a complete and optimized set of ICC reagents
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-102365
Réf. fabricant
15430984
Type de produit
Kit d’immunocytochimie
Quantité
40 Tests
À utiliser avec (application)
Analyse cellulaire, Imagerie cellulaire, Recherche sur les cellules souches, Analyse de cellules souches, Marquage et détection des cellules souches, Immunocytochimie (ICC)
Conjugué
2 couleurs, Alexa Fluor 488, Alexa Fluor 555
Forme
Liquide
Contenu et stockage
Stockage entre -20°C et 4°C :</li><li>10 µl de OCT4 de lapin anti-humain (200X)</li><li>20 µl de SSEA4 de souris anti-humain (100X)</li><li>20 µl d’Alexa Fluor™ 555 d’âne anti-lapin (250X)</li><li>20 µl d’Alexa Fluor™ 594 d’âne anti-lapin (250X)</li><li>20 µl d’Alexa Fluor™ 488 de chèvre anti-IgG3 de souris (250X)<br><br>Conserver à -20°C à TR :</li><li>1 flacon de réactif ReadyProbes™ de cellule fixe NucBlue™ (1X)</li><li>10 ml de solution fixative (1X)</li><li>10 ml de solution de perméabilisation S (1X)</li><li>20 ml de solution de blocage (1X)</li><li>20 ml de tampon de lavage (10X)
Contenus des kits
SSEA4 de souris anti-humain (100X), Alexa Fluor™ 555 singe anti-lapin (250X), Alexa Fluor™ 594 singe anti-lapin (250X), Alexa Fluor™ 488 anti-IgG3 de chèvre (250X), réactif Probes™ à cellules fixes NucBlue™ (1X), sol fixateur
Quantité d’emballage
1/boîte
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