Defined, low-insulin, serum- and animal-component free medium for the differentiation of human ES and iPS cells
Type de cellule
Pluripotent Stem Cells
À utiliser avec (application)
Cell Culture, Differentiation
Quantité
100 mL
Marque
STEMCELL Technologies
STEMdiff™ APEL™ 2-LI Medium is a fully defined, low-insulin (LI), serum-free and animal component-free medium for the differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells
It is based on the APEL formulation published by Dr
Andrew Elefanty and lacks undefined components such as protein-free hybridoma medium
STEMdiff™ APEL™ 2-LI can be used in adherent or embryoid body (EB)-based protocols, such as with AggreWell™
Appropriate induction factors must be added before use
The low insulin content of this medium makes it particularly useful for differentiation to lineages in which insulin is a known inhibitor, such as cardiomyocyte
