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Ref : 17158261

1713.00

For differentiation of human ES and iPS cells to monocytes

Type de cellule :

Dendritic Cells, Macrophages, Monocytes, Myeloid Cells, Pluripotent Stem Cells

À utiliser avec (application) :

Cell Culture, Differentiation, Expansion

Quantité :

1 Kit

Brand

STEMCELL Technologies

STEMdiff™ Monocyte Kit includes serum-free media and supplements for the feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to monocytes expressing CD14
The simple protocol is performed in 2D adherent cultures
During the first 3 days, Medium A induces cells toward mesoderm
For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B
At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes
CD14⁺ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period
Peak CD14⁺ frequency is typically 60 - 80%

Type de cellule : Dendritic Cells, Macrophages, Monocytes, Myeloid Cells, Pluripotent Stem Cells

À utiliser avec (application) : Cell Culture, Differentiation, Expansion

Quantité : 1 Kit

Gibco™ Milieu Neurobasal™-A, pas de D-glucose, pas de pyruvate de sodium 93.25

Gibco™ Milieu AIM V™, liquide (niveau de recherche) 196.00€ - 228.00€