STEMdiff™ Monocyte Kit includes serum-free media and supplements for the feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to monocytes expressing CD14
The simple protocol is performed in 2D adherent cultures
During the first 3 days, Medium A induces cells toward mesoderm
For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B
At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes
CD14+ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period
