The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
Caractéristiques techniques
| Marque | Invitrogen |
|---|
| Réf. B-LABO | BL-RE-106319 |
|---|
| Réf. fabricant | 18567964 |
|---|
| Numéro d’adhésion | 60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; Caga; Cagb; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; Chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK_01322; EGK_21438; GAGB; intracellular calcium-binding protein (MRP14); L1AG; Leukocyte L1 complex heavy chain; leukocyte L1 complex light chain; LIAG; LOC100735514; LOC532569 protein; MA387; MAC387; macrophage migration inhibitory factor-related protein-8; MIF; Migration inhibitory factor-related protein 14; migration inhibitory factor-related protein 8; Mrp14; MRP-14; Mrp8; MRP-8; Myeloid-related protein 14; neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; NIF; P14; p23; P7; P8; Pro-inflammatory S100 cytokine; protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9; S100 calcium bin |
|---|
| Plage de dosage | 0.65 to 150 ng/mL |
|---|
| Sensibilité du dosage | 0.65 ng/mL |
|---|
| Conjugué | Biotin |
|---|
| Type de produit | ELISA |
|---|
| Type d’échantillon | Supernatant |
|---|
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
|---|
| Identification génétique (Entrez) | P27005, P31725 |
|---|
| Symbole de gène(s) | S100A8, S100A9 |
|---|
| Coefficient de variation inter-dosage | <12% |
|---|
| Coefficient de variation intra-dosage | <10% |
|---|
| Contenus des kits | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
|---|
| Marqueur ou colorant | HRP |
|---|
| Quantité | 10 x 96 Tests |
|---|
| État réglementaire | Research Use Only |
|---|
| Volume d’échantillon | Serum, 50 µL; Plasma, 50 µL; Supernatant, 50 µL |
|---|
| Conditions de stockage | 2°C to 8°C |
|---|
| Cible | Calprotectin |
|---|
| Heure du test | 1 hr 20 min |
|---|
| Durée totale du dosage | 4 hr 45 min |
|---|
Une question, une configuration particulière ?
Contactez-nous pour un devis.
