Competitive ELISA
The Multispecies Estrone ELISA quantitates estrone in fecal extracts,urine,and tissue culture media.
Principle of the methodThe Estrone Competitive ELISA research-use-only kit is designed to quantitatively measure estrone present in extracted dried fecal samples,urine,and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone,estrone-3-sulfate,and estrone 3-glucuronide in urine and fecal samples with almost equal affinity,allowing for non-invasive testing of this steroid. An estrone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validationEach manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Estrone is an aromatized C18 steroid with a 3-hydroxyl group and a 17-ketone, a major mammalian estrogen. it is converted from androstenedione directly, or from testosterone via estradiol. In humans, it is produced primarily by the cyclic ovaries, placenta, and the adipose tissue of men and postmenopausal women. Therapeutic estrone is the synthetic form of a naturally occurring estrogen estrone. Estrone diffuses through the cell membrane and binds to and subsequently activates the nuclear estrogen receptor found in the reproductive tract, breast, pituitary, hypothalamus, liver, and bone. The activated hormone-receptor complex gets translocated to the nucleus where it binds to the estrogen response element on the dna and promotes the transcription of target genes involved in the functioning of the female reproductive system and secondary sex characteristics. Estrone increases the hepatic synthesis of sex hormone binding globulin (shbg), thyroid-binding globulin (tbg), and other serum proteins, as well as suppresses the release of follicle-stimulating hormone (fsh) and luteinizing hormone (lh) from the anterior pituitary. [National Center for Biotechnology Information. PubChem Compound Database; CID=5870].
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-106705 |
| Réf. fabricant | 17835117 |
| Plage de dosage | 31,25-2000 pg/ml |
| Sensibilité du dosage | 22,4 pg/ml |
| Doser | Dosage multiplex sur la plateforme Luminex |
| Conjugué | HRP |
| Méthode de détection | Fluorescent |
| Type de produit | Kit ELISA |
| Type d’échantillon | Extrait fécal, Surnageant, Urine |
| Suffisant pour | 10 x 96 dosages |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Coefficient de variation inter-dosage | 5,5% |
| Coefficient de variation intra-dosage | 4,7% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| État réglementaire | RUO |
| Disciplines de recherche | Biologie de la reproduction |
| Volume d’échantillon | Extrait fécal, 50 μl ; surnageant, 50 μl ; urine, 12,5 μl |
| Conditions de stockage | -20°C |
| Cible | Humaine |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 2 h 30 min |
